It enables the separation, identification and quantification of components in a mixture through a high-efficiency chromatographic column.
Benefits
The structure and operation of the instrument make it possible to:
- separate complex compounds with high resolution and accuracy
- analyze a wide range of substances, including natural and synthetic active ingredients and specific compounds to be determined
- obtain rapid results due to the high speed of analysis
- use very small amounts of sample
- improve reproducibility and accuracy of analysis through precise control of operating conditions
- realize accurate qualitative and quantitative analysis, with the ability to detect and quantify components at very low concentrations.
Structure
The main elements that make up the instrument are:
- high-pressure pump
- injector for sample introduction
- chromatographic column containing the stationary material
- detector to identify and quantify the separated components
- data management system for recording and analyzing results.
How it works
The sample to be analyzed consists of a mixture of compounds dissolved in a solvent. It is injected into the mobile phase (solvent) stream that is pumped through the chromatographic column at high pressure. The various compounds in the sample interact differently with the stationary phase inside the column, separating temporally as they leave the column. The separated compounds are detected by the detector, which generates signals proportional to the amount of each component. These signals are then analyzed and recorded by the data management system.
